果树组织培养中褐化现象的研究进展

介绍了果树植物组织培养褐变的发生机理，发生褐变的主要果树种类，综述了近年来果树植物组织培养中参与褐变现象的酚类物质、酚氧化酶以及多种影响因素的研究进展。     

Comparative study on antioxidative system in normal and vitrified shoots of Populus suaveolens in tissue culture

To explore the physiological and biochemical mechanism of the occurrence of vitrified shoots of Populus suaveolens in tissue culture, the changes in water, chlorphyll, lignin, H₂O₂, phenylalanine ammonialyase (PAL), malonaldehyde (MDA), protective enzymatic systems, and some key enzymes involved in the ascorbate- glutathione cycle were comparatively studied in both normal and vitrified shoots of P. suaveolens. The results show that the lower activities of peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), glutathione reductase (GR) and PAL, and the less contents of chlorphyll, lignin, ascorbate (ASA) and reduced glutathione (GSH) as well as the lower ratios of ASA / DHA and GSH / GSSG are observed in vitrified shoots than in normal ones during the whole culture period. While in comparison with normal shoots, the higher activity of superoxide dismutase (SOD) and the more concentrations of water, H₂O₂, MDA, dehydroascorbate (DHA) and oxidized glutathione (GSSG) are found in vitrified shoots. Statistical analysis indicates that the enhanced activity of SOD and the decreased activities of CAT and POD as well as some enzymes involved in the ascorbate-glutathione cycle might be closely correlated to the accumulation of H₂O₂. The less regeneration of ASA and GSH and the lower capacity of the ascorbate-glutathione cycle observed in vitrified shoots might be due to a significant decrease in APX, MDAR, DHAR and GR activities and a decline in redox status of ASA and GSH. The decreases in chlorphyll content might result in a decline in photosynthesis. The lower activities of POD and PAL could result in the decrease of lignin synthesis and cell wall ligination, which might be the key factor leading to the increase in water content. It is concluded that the deficiency of detoxification capacity caused by the lower capacity of the ascorbate-glutathione pathway and the decreased activity of protective enzymatic system might lead to the large accumulation of H₂O₂ and the enhancement of membrane lipid peroxidation, which might be the main cause leading to the occurrence of vitrifying shoots of P. suaveolens in tissue culture. [Supported by National Natural Science Foundation of China (Grant No. 30271093) and the Foundation of State-designated Base for Biology Researching and Teaching in Beijing Forestry University]     

柠条锦鸡儿组培苗继代培养中矿质元素的吸收规律

比较柠条锦鸡儿在MS、WPM、B5三种基本培养基中对P、K、Ca、Mg、Mn、Cu等主要矿质元素的吸收规律,用向量分析方法找出三种基本培养基中的限制因子.结果表明:矿质养分整体供应水平是影响柠条锦鸡儿组培苗吸收矿质元素的关键因子;除此之外,MS培养基的Ca、P水平偏低,WPM和B5培养基的主要限制因子分别为K、Mn和Ca、P、K.     

红叶石楠组培苗瓶外生根技术研究

以苗龄为40 d的红叶石楠组培继代芽苗为瓶外生根试验材料,采用多因素正交试验设计研究不同栽培基质、不同激素、不同激素浓度处理及处理时间对红叶石楠继代芽苗瓶外生根诱导的影响,继代芽苗扦插前的不同炼苗方式及扦插后施肥方式对芽苗瓶外生根率的影响。结果表明:A3B1C3D2组合(珍珠岩、生根粉ABT 200 mg.L-1浸泡处理30 min)获得59%瓶外生根率,而A3B1C1D2(珍珠岩、ABT 50 mg.L-1浸泡处理30 min)为最理想的组合;芽苗在自然散射光下炼苗15~20 d,生根率可提高到81%;栽后适时追施营养液有利于芽苗的高生长。继代芽苗瓶外生根技术的应用减少组培苗培养工序,促进红叶石楠的组培快繁效率,降低组培苗的生产成本。     

尾叶桉组织培养快速繁殖的研究

以顶芽或茎段为外植体 ,通过 4种基本培养基和生长调节剂不同浓度组合的对比试验 ,筛选出最佳增殖培养基为B1+2 0mg·L-16 -BA +1 0mg·L-1NAA ,生根培养基为B2 +0 5mg·L-1ABT1号。移栽基质选择红心土。     

不同种源翅荚木的抗寒性

以5个种源的翅荚木叶为材料,采用叶片离析法和石蜡制片技术观测叶片结构特征、电导率法测定其半致死温度、差示热量扫描仪测定其过冷点温度,并结合田间试验分析不同种源翅荚木苗期的抗寒性及其与叶片结构的关系.结果表明:湖南通道翅荚木种源抗寒性最强,苗期在湖南株洲无冻害,在浙江丽水冻害率为4.83%,极显著低于其他种源;其叶片半致死温度为-5.34 ℃,过冷点温度为-15.04 ℃,均低于广东翁源和广西忻城种源. 不同种源翅荚木叶片细胞结构紧密度(CTR)和疏松度(SR)与种源的抗寒性有一定的关系,CTR值越大、SR值越小,种源的抗寒性越强.     

红千层愈伤组织诱导及植株再生

以茎段、芽和叶片为材料,探讨了红千层愈伤组织诱导及植株再生的方法.结果表明:红千层的茎段、芽和叶片均可诱导出愈伤组织,通过继代培养可发育成绿色和粉红色2种类型的愈伤组织,其中绿色、致密的愈伤组织可以分化出不定芽;培养基1/2M S 6-BA 1.0 m g/L NAA 0.1 m g/L适宜愈伤组织不定芽的诱导,在培养基1/2M S IBA 0.25 m g/L上试管苗的生根率可达95%.     

福建比利时杜鹃叶片矿质营养含量的研究

分别用凯氏定氮法、钼蓝比色法、火焰光度法和原子吸收分光光度法,对市场上生长良好的福建比利时杜鹃花叶片N、P、K、Ca、Mg、Fe、Mn、Cu、Zn的含量进行了测定,并运用数理统计方法和SPSS软件对测定结果进行了分析.结果表明,未成熟叶片中各营养元素含量分别为N 32.9 g/kg, P 4.6 g/kg, K 22.6 g/kg, Ca 23.9 g/kg, Mg 3.4 g/kg,Fe 123.3 mg/kg,Mn 249.2 mg/kg, Cu 27.5 mg/kg,Zn 163.2 mg/kg.活性Fe的含量为12.3 mg/kg.N、P、K的比例为1∶0.14∶0.69.成熟叶片中营养元素含量分别为N 28.0 g/kg,P 4.2 g/kg1,K 24.2 g/kg,Ca 24.9 g/kg,Mg 3.6 g/kg,Fe 265.5 mg/kg,Mn 354.3 mg/kg,Cu 19.8 mg/kg,Zn 142.3 mg/kg.活性Fe的含量为18.0 mg/kg.N、P、K的比例为1∶0.15∶0.87.该分析结果可供其他地区生长不良的杜鹃进行营养诊断时参考,以改善杜鹃生长不良的状况.     

Liquid culture and transformation of hinoki cypress (Chamaecyparis obtusa Sieb. et Zucc.)

Hinoki cypress (Chamaecyparis obtusa) is one of the most important timber resource forest trees in Japan. Because seed production from a seed orchard of hinoki cypress is not constant every year, micropropagation from a limited amount of material is useful. Up to now, the conventional tissue culture method using solid medium has been used. Here a new method using liquid culture in tubes rotated vertically is described. Shoot primordium of hinoki cypress was inoculated in Campbell and Durzan’s (CD) liquid medium containing different cytokinins (6-benzylaminopurine (BAP), Zeatin, thidiazurone (TDZ)), and the container tubes were rotated vertically around the axis at 2 times / min. Culture room temperature was 25°C and light condition was 16 h photoperiod per day of fluorescent lamps. Zeatin at 1μM concentration was the best for maintaining the shoot primordium production and TDZ induced callus on the surface of the shoot primordia. After shoot primordium multiplication in the liquid culture, they were transplanted to agar medium for shoot elongation. A high concentration of agar (up to 16 g/L) or AVF (anti vitrification factor from Dr. Nairn, 1995) was effective to prevent vitrification of the shoots. Transformation of shoot primordium was done using particle bombardment with vectors containingβ-glucuronidase (GUS) gene or herbicide resistance gene (bar). Positive result for transient transformation was observed with the histo-chemical study for transformation with GUS. Integration of a useful herbicidebar gene into the shoot primordium culture system was also tried and stably transformed plants were obtained. This is the first report of stable transformation of Japanese conifer using practically useful gene. The generous supply of AVF-B from Dr. B.J. Nairn, Tasman Forestry, NZ is also appreciated.     

滩地淹水胁迫对杨树生长与木材显微结构的影响

对5种不同淹水时间类型滩地1～10年生杨树单株材积生长动态进行了分析.结果表明:不同类型之间单株材积生长量均存在显著差异,且随着林龄增长,其差异逐步减小;不同淹水胁迫下,当年年轮宽度、纤维细胞数量、木材基本密度均存在显著差异,纤维长度和宽度差异不显著;杨树年轮宽度与径向纤维细胞数、导管数、径向纤维比量呈正相关;随淹水胁迫程度加深,当年形成层细胞分裂受到了显著抑制,纤维细胞以及导管数量减少,林木径向生长量下降,径向导管比量提高,纤维比量下降,细胞壁腔比减小,木材密度明显降低.对5种淹水类型滩地的林木生长进行了系统聚类,合并划分为4种生长类型,对其中3种常规造林宜林类型杨树生长潜力进行了预测.